Part:BBa_J119016:Design

pLac-RBS-T7RNAP-pT7-RBS-RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 3417
  Illegal AgeI site found at 3529
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 126
  Illegal BsaI.rc site found at 99
  

Design Notes

The gene produces a red color but we have speculated that this is caused by read through transcription.

Source

The pLac-RBS-T7RNAP gene came from geneart DNA synthesis. The pT7-RBS-GFP came from iGEM09_MoWestern_Davidson.